Suitable for use in FLISA and Immunofluorescence. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Absorption Wavelength: 495nm Emission Wavelength: 528nm Fluorochrome/Protein Ratio: 1.7 moles FITC per mole of Goat IgG F(ab’)2
Storage and Stability
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Guinea Pig IgG whole molecule
Form
Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Label: Fluorescein isothiocyanate (FITC) (MW 390 daltons) Reconstitution: 1.0ml sterile dH2O.
Purity
Purified by immunoaffinity chromatography.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, guinea pig IgG and guinea pig Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).