Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.

Immunohistochemistry: cytostat sections Optimal dilutions to be determined by the researcher.

Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Human IgG1 (Fc portion) from human serum.

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).

Purified by Protein G affinity chromatography.

Recognizes human IgG1 (kappa and lambda light chain), Fc region specific. No crossreactivty was found against IgG2, IgG3, IgG4, IgM, IgA or IgD.