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I1904-75L1-ML550 Rat Anti-IgG1 (MaxLight 550)

Specifications
References
Brand
MaxLight™
Clone Type
Monoclonal
Host
Rat
Source
Mouse
Conjugate
MaxLight™550
Isotype
IgG1
Clone Number
3H2301 (LO-MG1-13)
Grade
Affinity Purified
Applications
FLISA
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free

MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.

Applications
Suitable for use in FLISA. Other applications have not been tested.
Recommended Dilutions
Optimal working dilutions to be determined by researcher.
Hybridoma
Spleen cells from immunized LOU/c rats were fused with cells of the rat IR983F myeloma cell line.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Purified mouse IgG1 from BALB/c mice.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.
Purity
Purified by affinity chromatography from tissue culture supernatant.
Specificity
Recognizes the gamma 1 heavy chain of mouse immunoglobulin. No crossreactivity with other immunoglobulin classes or subclasses. Does not crossreact with human.
References
1. Denis O., Latinne, D., Nisol, F. and Bazin, H. (1993). Resting B cells can act as antigen presenting cells in vivo and induce antibody responses. Int. Immunol. 5: 71-78. 2. Soares, M. et al. (1992) Isotype characterization of rat preformed natural antibodies against guinea pig cells. Transplantation Proceedings 24: 51-452.
USBio References
No references available
Conjugates
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