Suitable for use in FLISA. Other applications not tested.
Recommended Dilutions
Optimal working dilutions to be determined by researcher.
Hybridoma
Sp2/0 myeloma cells with spleen cells from BALB/c mice.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Rat IR27 IgG1 myeloma protein
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by affinity chromatography from tissue culture supernatant.
Specificity
Recognizes the heavy chain of rat IgG1. No crossreactivity is observed with other rat immunoglobulin classes or subclasses.
References
1. Pelegri, C., et al., Clin. Exp. Immunol. 125: 470-477 (2001). 2. Sato, K., et al., J. Immunol. 166: 4185-4194 (2001). 3. Bezie S., et al., PLoS One. 10 (3): e0119686 (2015). 4. Bezie S., et al., Journal of Immunology 195(12):5805-15 (2015).