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I1904-80F Mouse Anti-IgG2

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
P01859
Isotype
IgG1,k
Clone Number
3H2343 (HP6014)
Grade
Purified
Applications
E IHC
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

Applications
Suitable for use in Immunohistochemistry and ELISA. Other applications have not been tested.
Recommended Dilutions
Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies
I1904-65W: IgG, F(ab’)2 (HRP) Pab RbxMo I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab RbxMo
Recommended Negative Control
I1904-78R: IgG1 Murine Negative Control
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified human IgG2 myeloma protein.
Form
Supplied as a liquid in 100mM borate buffered saline, pH 8.2. No preservatives added.
Purity
Purified.
Specificity
Recognizes human IgG2 at an epitope localized to the Fab region. Will not cross-react with IgG1, IgG3, IgG4, free kappa, free lambda, IgM, IgA1, IgA2 or IgD as tested by hemagglutination assays.
References
1. Jefferis, R. et al. (1985). Evaluation of Monoclonal Antibodies having specificity for Human IgG sub-classes. Results of an IUIS/WHO collaborative study. Immunology Letters. 10: 223-252.2. Hussain, R. et al. (1986). 2. Hussain, R. et al . (1986). Use of monoclonal antibodies to quantify subclasses of human IgG. J. Immunol. Meth. 93: 89-96. 3. Jefferis, R. et al . (1988). Immunology. 63: 171.
USBio References
No references available
Conjugates
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