Suitable for use in ELISA. Other applications not tested.
Recommended Dilutions
ELISA: 1-2ug/ml (Use as detection antibody). Optimal working dilutions to be determined by researcher.
Fusion Partners
Spleen cells from immunized LOU/c rats were fused with cells of the rat IR983F myeloma cell line.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified IgG from BALB/c mice.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Reacts with mouse IgG2b heavy chain.
References
1. Bazin, H. et al. (1984). Rat monoclonal antibodies. . Rapid purification from in vitro culture supernatants. J. Immunol. Meth. 71: 261-269.
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