Suitable for use in FLISA. Other applications not tested.

FLISA: 5ug/ml (as a coating antibody) Optimal working dilutions to be determined by researcher.

Avidity for rat IgG2b: 4.7 x 10e9 M-1.

Fusion Partners: Spleen cells from immunized BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.

Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Rat IgG2b myeloma protein (IR863).

Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).

Purified by affinity chromatography.

Recognizes the rat IgG2b heavy chain. Does not crossreact with other immunoglobulin classes or subclasses.