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I1904-84H-ML650 Mouse Anti-IgG2b (MaxLight 650)

Specifications
References
Brand
MaxLight™
Clone Type
Monoclonal
Host
Mouse
Source
Rat
Conjugate
MaxLight™650
Isotype
IgG1
Clone Number
MARG 2b-8
Grade
Affinity Purified
Applications
FLISA
Crossreactivity
Rt
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free

MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.

Applications
Suitable for use in FLISA. Other applications not tested.
Recommended Dilutions
FLISA: 5ug/ml (as a coating antibody) Optimal working dilutions to be determined by researcher.
Avidity for rat IgG2b: 4.7 x 10e9 M-1.
Fusion Partners: Spleen cells from immunized BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight 650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Rat IgG2b myeloma protein (IR863).
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
Purity
Purified by affinity chromatography.
Specificity
Recognizes the rat IgG2b heavy chain. Does not crossreact with other immunoglobulin classes or subclasses.
References
1. Sato, K., et al., Carbon monoxide generated by heme oxygenase-1 suppresses the rejection of mouse-to-rat transplant. J. Immunol. 166: 4185-4194 (2001).
USBio References
No references available
Conjugates
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