Suitable for use in ELISA. Other applications not tested.

ELISA: 5ug/ml (as a coating antibody) Optimal working dilutions to be determined by researcher.

Avidity for rat IgG2b: 4.7 x 10e9 M-1.

Fusion Partners: Spleen cells from immunized BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.

May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Rat IgG2b myeloma protein (IR863).

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

Purified by affinity chromatography.

Recognizes the rat IgG2b heavy chain. Does not crossreact with other immunoglobulin classes or subclasses.