Suitable for use in ELISA. Other applications have not been tested.
Recommended Dilutions
ELISA: 5ug/ml (coating) Optimal dilutions to be determined by the researcher.
Hybridoma
Spleen cells from immunized Lou/c rats were fused with cells of the rat IR983F myeloma cell line.
Matched Pair
Capture: I1904-86 Detection: I1904-86D
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Polyclonal mouse IgG
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by affinity chromatography.
Specificity
Recognizes mouse IgG3 (gamma heavy chain) and does not crossreact with other mouse immunoglobulin classes or subclasses.
References
1.Denis O et al., Int Immunol 5:71-78 (1993) 2.Savignac M et al., J. Immunol 185:7527-36 (2001)
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