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I1904-89B1 Mouse Anti-IgG4

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
P01861
Isotype
IgG1
Clone Number
3H2426 (HP6025)
Grade
Purified
Applications
E IHC WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions
Immunohistochemistry: Frozen and paraffin sections. Optimal dilutions to be determined by the researcher.
Hybridoma
SP2/0-Ag14 myeloma cells with spleen cells from Balb/c mice.
Recommended Secondary Antibodies
I1904-65W: IgG, F(ab’)2 (HRP) Pab RbxMo I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab RbxMo
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified human IgG4.
Form
Supplied as a liquid in borate buffered saline, 0.09% sodium azide.
Purity
Purified
Specificity
Recognizes the heavy chain of human IgG4 at an epitope in the Fc region. Does not crossreact with IgG1, IgG2, IgG3, IgM or IgA. Species Crossreactivity: chimpanzee
References
1. Jefferis, R. et al. (1985). Immunology Letters. 10:223-252. 2. Agaimy, A. et al. (2010) Am J Surg Pathol. 34:271-8. 3. Strehl, J.D. et al. (2011) Numerous IgG4-positive plasma cells are ubiquitous in diverse localised non-specific chronic inflammatory conditions and need to be distinguished from IgG4-related systemic disorders. J Clin Pathol. Jan 12. 4. Black, C.M. et al. (1991) Immunology. 72:94-8. 5. Fernandez-Becerra, C. (2010) Malar J. 9:29. 6. Lee, H.W. et al. (2008) Korean J Parasitol. 46:195-8.
USBio References
No references available
Conjugates
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