Applications
Suitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:2000-1:10,000 Western Blot: 1:500-1:3000 Immunohistochemistry: 1:200-1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing alkaline phosphatase conjugates will result in a substantial loss of activity.
Immunogen
Monkey IgM mu heavy chain.
Form
Supplied as a liquid in 0.05M Tris Chloride, 0.15M sodium chloride, 0.001M magnesium chloride, 0.0001M zinc chloride, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide 50% glycerol, pH 8.0. Labeled with Alkaline Phosphatase (AP), MW: 140kD.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes monkey IgM. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine), anti-goat serum, monkey IgM and monkey serum. Cross-reacts against other mouse or human heavy or light chain isotypes; <1%.