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I1905-15I-ML550 Mouse Anti-IgM (MaxLight 550)

Specifications
References
Brand
MaxLight™
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
P01871
Conjugate
MaxLight™550
Isotype
IgG1
Clone Number
3H2438 (CM7)
Grade
Affinity Purified
Applications
FC FLISA IHC WB
Crossreactivity
Ca Eq Fe Hu Mk Po
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free

MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.

Applications
Suitable for use in Flow Cytometry, FLISA, Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions
Immunohistochemistry: Paraffin sections. Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Purified human IgM, and then purified feline IgM.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.
Purity
Purified by Protein A affinity chromatography from tissue culture supernatant.
Specificity
Recognizes human IgM. No crossreactivity with other immunoglobulin classes or sub classes. Species Crossreactivity: Rhesus and marmoset monkey, equine, feline, canine, porcine
References
1. Reubel, G.H. et al. (1992). Interaction of acute feline Herpes virus - 1 and chronic feline immunodeficiency virus infections in experimentally infected specific pathogen free cats. Vet. Immunol. Immunopathol. 35: 95-119. 2. Lunn, D.P. et al. (1998) Report of the Second Equine Leucocyte Antigen Workshop, Squaw valley, California, July 1995. Vet Immunol Immunopathol. 62 (2): 101-43. 3. Chandhasin, C. et al. (2005) Unique long terminal repeat and surface glycoprotein gene sequences of feline leukemia virus as determinants of disease outcome. J Virol. 79 (9): 5278-87. 4. Prieto, J.M. et al. (2017) Developmental expression of B cell molecules in equine lymphoid tissues. Vet Immunol Immunopathol. 183: 60-71. 5. Tallmadge, R.L. et al. (2012) Expression of essential B cell development genes in horses with common variable immunodeficiency Molecular Immunology. 51 (2): 169-76.
USBio References
No references available
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