Suitable for use in ELISA, Flow Cytometry and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions
ELISA: 1:100-1:1000 Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified canine IgM
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide
Purity
Purified by affinity chromatography
Specificity
Recognizes canine IgM and shows no crossreactivity with other canine immunoglobulin classes in immunoelectrophoresis and ELISA. May crossreact with IgM from other species.
References
1. Peters, I. R. et al.. Clin. Diag. lab. immunol. 11; 841-848.(2004).