Suitable for use in Flow Cytometry, Immunohistochemistry, FLISA, Western Blot and Immunofluorescence. Other applications have not been tested.

Immunohistochemistry (Frozen, paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin embedded sections. Sodium citrate buffer, pH 6.0 is recommended. Western Blot (Reducing conditions): Detects a band of 50-56kD that represents the nucleocapsid. Also detects specific bands at 56 and 46kD in reducing gels with canine coronavirus (CCV) as the antigen. Optimal dilutions to be determined by the researcher.

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. 

Note: Applications are based on unconjugated antibody.

Coronavirus cocktail

Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.

Purified by Protein A affinity chromatography from tissue culture supernatant.

Recognizes feline infectious peritonitis virus (FIPV) type 1 and 2. Specific for the nucleocapsid. Shown to react with canine coronavirus (CCV), porcine coronavirus transmissable gastroenteritis virus (TGEV) and ferret coronavirus. Some reactivity has been observed against bovine coronavirus (BCV). Exhibits negative reactivity against feline leukemia virus, feline immunodeficiency virus, feline calcivirus, feline Herpes virus, canine adenovirus (type 2), canine distemper virus, canine parvovirus and canine parainfluenza virus.