USBio Logo

I7661-02B Rabbit Anti-Insulin-Like Growth Factor I (IGF1, IGF I, IGF-I, IBP1, Mechano Growth Factor, MGF, Somatomedia C, Somatomedin C)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
P05019
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
Insulin-like Growth Factor IA, IGF-IA, IGF1A, Insulin-like Growth Factor IB, IGF-IB, IGF1B

Growth Factor I, a 70aa secreted mitogenic polypeptide and member of the insulin gene family, produced primarily by the liver. IGFI exhibits both autocrine and paracrine properties and has a widespread effect on cells including those of the bone, cartilage, muscle, nerves, skin, liver and lungs, playing a key role in cell proliferation and growth, inhibition of apoptosis and linked with tumor cell growth.

IGFI signals through binding to the type 1 insulinlike growth factor receptor (IGF1R) and also to the insulin receptor (IR), resulting in the activation of the AKT signalling pathway. Production of IGFI is stimulated by growth hormone (GH) and can be hindered by GH insensitivity, deficiency in GH receptors or a malfunction in the post GH receptor signalling pathway.
Applications
Suitable for use in ELISA, Functional Assays, and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 0.5-2ug/ml Western Blot: 0.1-0.2ug/ml Functional Assays: 0.67-1ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant human IGFI.
Form
Supplied as a lyophilized powder from PBS, pH 7.2. No preservative added. Reconstitute with 100ul sterile ddH2O.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human IGFI.
References
1. Singer, C.F. et al. (2004) Insulinlike growth factor (IGF)I and IGFII serum concentrations in patients with benign and malignant breast lesions: Free IGFII is correlated with breast cancer size. Clin. Can. Res. 10: 40034009.
USBio References
No references available
Conjugates
Pricing
Order
Proceed to Checkout
Cart Summary
ProductSizeListYour PriceQtyExt Price
Subtotal:Subtotal:
Subtotal:Subtotal:
Total Coupon Savings:Total Coupon Savings:()
Your cart is currently empty.
- Coupon Code
Recently Viewed
Contact Us

Visit our technical library or contact our support staff to answer your questions.

Telephone:
1-800-520-3011

Library | Contact

Distributors

For customers outside of the United States, please use one of our many distributors.

View Distributors

Payment Methods

We accept the following payment methods as well as pay-by-invoice.

MasterCard Visa PayPal
© 2023-2024 United States Biological - All Rights Reserved