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I7661-05-ML650 Mouse Anti-Insulin-Like Growth Factor I (IGF1, Somatomedin C) (MaxLight 650)

Specifications
References
Brand
MaxLight™
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
P05019
Conjugate
MaxLight™650
Isotype
IgG1,k
Clone Number
0.T.61
Grade
Affinity Purified
Applications
IHC IP N WB
Crossreactivity
Hu Mo Rt
Accession #
NP_000609
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free

MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.

Insulin-like growth factor 1 (IGF-1) is a polypeptide protein hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. IGF-1 consists of 70 amino acids in a single chain with three intramolecular disulfide bridges. IGF-1 is produced primarily by the liver as an endocrine hormone and targets tissues in a paracrine/ autocrine fashion. Production is stimulated by growth hormone and can be retarded by undernutrition, growth hormone insensitivity, lack of growth hormone receptors, or failures of the downstream signaling pathway post GH receptor including SHP2 and STAT5b. Approximately 98% of IGF-1 is always bound to one of 6 binding proteins (IGF-BP). IGFBP-3, the most abundant protein, accounts for 80% of all IGF binding. IGF-1 binds to IGFBP-3 in a 1:1 molar ratio. Its primary action is mediated by binding to specific IGF receptors present on many cell types in many tissues. The signal is transduced by intracellular events. IGF-1 is one of the most potent natural activators of the AKT signaling pathway, a stimulator of cell growth and multiplication and a potent inhibitor of programmed cell death.
Applications
Suitable for use in Western Blot, Immunoprecipitation, Neutralization and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Immunohistochemistry: Formalin-fixed, paraffin-embedded skin sections. Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Human insulin-like growth factor I (IGF1) purified from human plasma. Species Sequence Homology: chicken
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human Insulin-Like Growth Factor I (IGF1), Mr ~17kD (migrates to ~7kD). Crossreactivity with IGF-II is ~40%. |Species Crossreactivity: mouse and rat. Occasional difficulty neutralizing rat IGF1 due to binding protein interference.
References
1. Harvey, A., et al., J. BIol. Chem. 274: 23,249-23,255 (1999). 2. Manes, S., et al., J. Biol. Chem. 274: 6935-6945 (1999). 3. Russell, W.E., et al., Proc. Natl. Acad. Sci. USA. 81: 2389-2392 (1984). 4. van Wyk, J.J., et al., Human Growth Hormone 585-599, Plenum Publishing Corp, NY (1986). 5. van Wyk, J.J., et al., Endocrinology 138: 4521 (1997). 6. Liu, et al., Molc. Cell. Neurosci. 5: 418-429 (1994).
USBio References
No references available
Conjugates
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