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I7663-04 Goat Anti-ILF2 (NF45, Interleukin enhancer binding factor 2, MGC8391, NF45, PRO3063, Nuclear factor of activated T-cells 45-D)

Specifications
References
Clone Type
Polyclonal
Host
Goat
Source
Human
Swiss Prot
Q12905
Isotype
IgG
Grade
Affinity Purified
Applications
E IF WB
Crossreactivity
Hu
Accession #
NP_004506.2
Shipping Temp
Blue Ice
Storage Temp
-20°C
ILF2; interleukin enhancer binding factor 2, 45kDa; MGC8391; NF45; PRO3063; interleukin enhancer binding factor 2; nuclear factor of activated T-cells, 45-kDa

Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of the interleukin 2 gene. NFAT binds to a sequence in the interleukin 2 gene enhancer known as the antigen receptor response element 2. In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the smaller of which is the product of this gene. The encoded protein binds strongly to the 90 kDa protein and stimulates its ability to enhance gene expression.

Applications
Suitable for use in ELISA, Western Blot, Immunofluorescence. Other applications not tested.
Recommended Dilution
Peptide ELISA Titer: 1:4000 Western Blot: 0.3-1ug/ml. ~45kD band observed in Human Tonsil and Thymus lysates. Immunofluorescence: 2-3ug/ml. Paraformaldehyde-fixed stable knock-down cell line (second half) shows merely background compared to the wildtype cell line (left half). Data kindly provided by Dr. A Antes, UMDNJ, Newark, USA. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Peptide Blocking
Corresponding peptide is available for Peptide Blocking studies. See I7663-04-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
Immunogen
Synthetic peptide corresponding to C-EKAYEKPPEKKEG, from the internal region (near the C Terminus) of thuman ILF2 (NP_004506.2).
Form
Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Purity
Purified by peptide affinity chromatography.
Specificity
Recognizes human Interleukin enhancer binding factor 2. Species sequence homology: mouse, rat, canine.
References
A protein interaction network for pluripotency of embryonic stem cells., Wang J, Rao S, Chu J, Shen X, Levasseur DN, Theunissen TW, Orkin SH. , Nature. 2006 Nov 16;444(7117):364-8. Epub 2006 Nov 8.
USBio References
No references available
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