CD25 is expressed by activated T lymphocytes and activated B lymphocytes responding to antigen or mitogen stimulation. CD25 is also expressed in some thymocytes and oligodendrocytes. In disease, elevated expression of CD25 in noted in a number of chronic inflammatory conditions, tuberculoid leprosy patients demonstrate markedly elevated levels of circulating CD25high FoxP3+ regulatory T cells (T-regs).

Suitable for use in Flow Cytometry. Other applications not tested.

Flow Cytometry: Neat; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher.

P3X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice

Store product at 4°C before and after reconstitution. DO NOT FREEZE! Lyophilized and reconstituted products are stable for 12 months at 4°C after receipt. Reconstitute lyophilized powder with sterile ddH2O prior to use and store remainder as an undiluted liquid. Caution: PE conjugates are sensitive to light. For maximum recovery of product centrifuge the original vial prior to removing the cap.

Human PHA blasts, day 3 of culture

Supplied as a lyophilized powder from PBS, pH 7.4, 1% BSA, 0.09% sodium azide, 5% sucrose. Labeled with R-Phycoerythrin (PE). Reconstitute with 1ml sterile ddH2O.

Purified by Protein A affinity chromatography

Recognizes the ~55kD alpha subunit of human IL-2Ra