IL-10 is a 18.7kD cytokine which exists as a homodimer. IL-10 is produced by a population of T-cells and is reported to inhibit the synthesis of several cytokines including IFN gamma, IL-2, IL-3 and TNF alpha.
Applications
Suitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended Dilutions
ELISA: 0.5-2ug/ml Western Blot: 0.1-0.2ug/ml; Reducing or non-reducing conditions. Optimal working dilutions to be determined by researcher.
Matched Pair
Capture: I8432-13C Detection: I8432-13A1
Recommended Positive Control
I8432-02R: Interleukin 10, Recombinant, Rat (Interleukin-10, IL-10, IL10, IL10X, Cytokine Synthesis Inhibitory Factor, CSIF)
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to rat IL-10, expressed in E. coli
Form
Supplied as a lyophilized powder from PBS. Reconstitute with 100ul sterile ddH2O.
Purity
Purified by immunoaffinity chromatography
Specificity
Recognizes rat interleukin-10 (IL-10).
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