MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.
IL-10 is a 18.7kD cytokine which exists as a homodimer. IL-10 is produced by a population of T-cells and is reported to inhibit the synthesis of several cytokines including IFN gamma, IL-2, IL-3 and TNF alpha.
Applications
Suitable for use in FLISA and Western Blot. Other applications have not been tested.
Recommended Dilutions
FLISA: 0.5-2ug/ml Western Blot: 0.1-0.2ug/ml; Reducing or non-reducing conditions. Optimal working dilutions to be determined by researcher.
Matched Pair
Capture: I8432-13C Detection: I8432-13A1
Recommended Positive Control
I8432-02R: Interleukin 10, Recombinant, Rat (Interleukin-10, IL-10, IL10, IL10X, Cytokine Synthesis Inhibitory Factor, CSIF)
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Recombinant protein corresponding to rat IL-10, expressed in E. coli
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
Purity
Purified by immunoaffinity chromatography
Specificity
Recognizes rat interleukin-10 (IL-10).