USBio Logo
Subscribe to our newsletter!

REGISTER | SIGN IN

Welcome | LOG OUT

J1255 Mouse Anti-J Chain

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
3H2560 (Mc19-9)
Grade
Affinity Purified
Applications
E IHC IP WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

J Chain serves to link two monomer units of either IgM or IgA. In the case of IgM, the J chain-joined dimer is a nucleating unit for the IgM pentamer, and in the case of IgA it induces larger polymers. It also help to bind these immunoglobulins to secretory component.

Applications
Suitable for use in Immunohistochemistry, ELISA, Immunoprecipitation and Western Blot. Other applications have not been tested.
Recommended Dilutions
Immunohistochemistry (Frozen): 1:200 ELISA: 5ug/ml Immunoprecipitation: 20ug/ml Optimal working dilutions to be determined by researcher.
Hybridoma
NS1 myeloma cells with spleen cells from Balb/c mice.
Recommended Secondary Antibodies
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-14C: IgG, Fc (HRP) Pab Gt xMo I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt xMo
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human J Chain.
Form
Supplied as a liquid in TBS, pH 7.0, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography from ascites.
Specificity
Recognizes human J Chain as tested by ELISA using purified human Ig fragments as the solid phase target antigen. Does not detect J chain in intact IgM in ELISA assays due to steric hindrance. In Western Blot J1255 detects J chain in IgM only in reducing conditions.
References
1.Arnold, J N et al., J Biol. Chem 280: 29080-29087
USBio References
No references available
Conjugates
Pricing
Order
Proceed to Checkout
Cart Summary
ProductSizeListYour PriceQtyExt Price
Subtotal:Subtotal:
Subtotal:Subtotal:
Total Coupon Savings:Total Coupon Savings:()
Your cart is currently empty.
- Coupon Code
Recently Viewed
Special Offers
30% OFF New Lab Discount Setting up a New Lab?
Apply for our New Lab Discount!
*Receive a 30% off LIST price coupon code via email.
Society Member Discount Members of the GSA, FASEB, ACS and SFN get even bigger discounts!
  • Earn Research Credit Try any antibody for an unspecified use. We'll reward you for your efforts!
    *Receive a 25% off list price coupon code.
    • Contact Us

    Visit our technical library or contact our support staff to answer your questions.

    Telephone:
    1-800-520-3011

    Library | Contact

    Site Map

    ANTIBODIES

    BIOCHEMICALS

    BIOLOGICALS

    CULTURE MEDIA

    MOLECULAR BIOLOGY

    KITS AND ASSAYS

    Technical Resources

    BIO DICTIONARY

    CITEAB

    ENTREZ GENE

    GENBANK

    HISTOLOGY GUIDE

    NCBI

    OMIM

    PROTEIN RESOURCES

    PROTOCOLS

    PUBMED

    UNIPROT

    Distributors

    For customers outside of the United States, please use one of our many distributors.

    View Distributors

    Payment Methods

    We accept the following payment methods as well as pay-by-invoice.

    MasterCard Visa PayPal
    © 2023-2024 United States Biological - All Rights Reserved