J Chain serves to link two monomer units of either IgM or IgA. In the case of IgM, the J chain-joined dimer is a nucleating unit for the IgM pentamer, and in the case of IgA it induces larger polymers. It also help to bind these immunoglobulins to secretory component.

Suitable for use in Immunohistochemistry, FLISA, Immunoprecipitation and Western Blot. Other applications have not been tested.

Immunohistochemistry: Frozen sections Optimal working dilutions to be determined by researcher.

NS1 myeloma cells with spleen cells from Balb/c mice.

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Human J Chain.

Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.

Purified by Protein A affinity chromatography from ascites.

Recognizes human J Chain as tested by ELISA using purified human Ig fragments as the solid phase target antigen. Does not detect J chain in intact IgM in ELISA assays due to steric hindrance. In Western Blot J1255 detects J chain in IgM only in reducing conditions.