Keratins are resolved with two dimensional gel electrophoresis into acidic pH, <5.7, and basic, pH >6.0 subfamilies. Members of the acidic and basic subfamilies are found together in pairs. The composition of keratin pairs varies with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
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