Potassium channel Kv1.2 is one of many different types of potassium channels in the cell. The Kv family of potassium channels seem to have a conserved homotetramer formation consisting of four voltage sensors and one pore domain. Kv1.2 cooperates with cortactin (an actin cytoskeleton-binding protein) and together they may have a part in the regulation of the ionic current.
Applications
Suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions
Western Blot: 0.5ug/ml detects Kv1.2 on 10ug of rat brain membrane tissue lysate. Immunohistochemistry (Paraffin): 1:500. Heat-induced epitope retrieval in citrate buffer, pH 6.0 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to rat Kv1.2. Cellular Localization: Membrane; Multi-pass membrane protein
Form
Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes rat Kv1.2.
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