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L3180-01H Rabbit Anti-LIVIN, CT (Baculoviral IAP Repeat-containing Protein 7, Kidney Inhibitor Of Apoptosis Protein, KIAP, Melanoma Inhibitor Of Apoptosis Protein, ML-IAP, Livin, RING Finger Protein 50, KIAP, BIRC7, MLIAP, RNF50, UNQ5800/PRO19607/PRO21344)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
Q96CA5-2
Isotype
IgG
Grade
Purified
Applications
E IHC WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

BIRC7 is a member of the family of inhibitor of apoptosis proteins (IAP) and contains a single copy of a baculovirus IAP repeat (BIR) as well as a RING-type zinc finger domain. The BIR domain is essential for inhibitory activity and interacts with caspases, while the RING finger domain sometimes enhances antiapoptotic activity but does not inhibit apoptosis alone. Two transcript variants encoding different isoforms have been found for this gene. The two isoforms have different antiapoptotic properties, with isoform alpha protecting cells from apoptosis induced by staurosporine and isoform b protecting cells from apoptosis induced by etoposide.

Applications
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:1,000 Immunohistochemistry: 1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide located within the aa region 271-298 in the C-terminus of human BIRC7.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation
Specificity
Recognizes human BIRC7.
References
1.Clark, H.F., et al., Genome Res. 13(10):2265-2270 (2003). 2.Gazzaniga, P., et al., Ann. Oncol. 14(1):85-90 (2003). 3.Vucic, D., et al., J. Biol. Chem. 277(14):12275-12279 (2002). 4.Sanna, M.G., et al., Mol. Cell. Biol. 22(6):1754-1766 (2002). 5.Kasof, G.M., et al., J. Biol. Chem. 276(5):3238-3246 (2001).
USBio References
No references available
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