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L4595-50C Rabbit Anti-LSD1 (Lysine-Specific Histone Demethylase, Amine Oxidase Flavin-containing Domain Protein 2, AOF2)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
O60341
Isotype
IgG
Grade
Affinity Purified
Applications
E FC IF IHC IP WB
Crossreactivity
Hu Mk Mo Rt
Gene ID
23028
Shipping Temp
Blue Ice
Storage Temp
-20°C

Lysine-specific demethylase 1 (LSD1; also known as AOF2 and BHC110) is a nuclear homolog of amine oxidases and functions as a histone demethylase and transcription cofactor (1). Gene activation and repression is specifically regulated by the methylation state of distinct lysine residues in histone proteins. For example, methylation of histone H3 Lys4 facilitates transcriptional activation by coordinating the recruitment of BPTF, a component of the NURF chromatin remodeling complex, and WDR5, a component of multiple histone methyltransferase complexes (2,3). In contrast, methylation of histone H3 (Lys9) facilitates transcriptional repression by recruiting HP1 (4,5). LSD1 is a component of the CoREST transcriptional co-repressor complex, which contains CoREST, CtBP, HDAC1 and HDAC2. As part of this complex, LSD1 demethylates mono-methyl and di-methyl histone H3 Lys4 via an FADdependent oxidation reaction, and facilitates the repression of neuronal-specific genes in non-neuronal cells (1,6,7). In contrast, when LSD1 is associated with androgen-receptor in human prostate cells, it demethylates mono-methyl and di-methyl histone H3 Lys9 and facilitates androgen-receptordependent transcriptional activation (8). Therefore, LSD1 can function as a co-repressor or co-activator, depending on gene context. LSD1 activity is inhibited by the amine oxidase inhibitors pargyline, deprenyl, clorgyline and tranylcypromine (8).

Applications
Suitable for use in Immunofluorescence/Immunocytochemistry, Flow Cytometry, ChIP, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Storage and Stability
Western Blot: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:200 IHC Protocol: Citrate/TBST Immunofluorescence (IC): 1:400 Chromatin IP: 1:50 Flow Cytometry: 1:400 Optimal dilutions to be determined by the researcher. May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to the C-terminus of human LSD.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA and 50% glycerol.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes endogenous levels of total human LSD1 protein. Species crossreactivity: mouse, rat and monkey.
References
1. Shi, Y. et al. (2004) Cell 119:941–953. 2. Wysocka, J. et al. (2006) Nature 442:86–90. 3. Wysocka, J. et al. (2005) Cell 121:859–872. 4. Jacobs, S.A. and Khorasanizadeh, S. (2002) Science 295:2080–2083. 5. Nielsen, P.R. et al. (2002) Nature 416:103–107. 6. Shi, Y.J. et al. (2005) Mol. Cell 19:857–864. 7. Lee, M.G. et al. (2005) Nature 437:432–435. 8. Metzger, E. et al. (2005) Nature 437:436–439.
USBio References
No references available
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