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L5999-06A-ML490 Mouse Anti-Luciferase, photinus pyralis (MaxLight 490)

Specifications
References
Brand
MaxLight™
Clone Type
Monoclonal
Host
Mouse
Source
Firefly
Swiss Prot
P08659
Conjugate
MaxLight™490
Isotype
IgG1
Clone Number
3H2605 (LUC-Y)
Grade
Affinity Purified
Applications
FC FLISA IF IHC WB
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free

MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.

Applications
Suitable for use in Flow Cytometry, Immunohistochemistry, Immunofluorescence, FLISA and Western Blot. Other applications have not been tested.
Recommended Dilutions
Flow Cytometry: Membrane permeabilization is required Immunohistochemistry: Frozen sections Optimal working dilutions to be determined by researcher.
Recommended Negative Control
I1904-78R: IgG1 Murine Negative Control
Hybridoma
Sp20 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Recombinant protein corresponding to Luciferase expressed in E. coli.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.
Purity
Purified by Protein A affinity chromatography from tissue culture supernatant.
Specificity
Recognizes the luciferase gene of the North American firefly Photinus pyralis in cells and tissues.
References
1. Laakkonen, J.P. et al. (2009) Clathrin-independent entry of baculovirus triggers uptake of E. coli in non-phagocytic human cells. PLoS One. 4:e5093. 2. van Beusechem, V.W. et al. (2002) Efficient and selective gene transfer into primary human brain tumors by using single-chain antibody-targeted adenoviral vectors with native tropism abolished. J Virol. 76: 2753-62. 3. Zhao, D. et al. (2008) Antivascular effects of combretastatin A4 phosphate in breast cancer xenograft assessed using dynamic bioluminescence imaging and confirmed by MRI. FASEB J. 22: 2445-51. 4. Spiotto, M.T. et al. (2010) Imaging the unfolded protein response in primary tumors reveals microenvironments with metabolic variations that predict tumor growth. Cancer Res. 70: 78-88. 5. Howarth, J.L. et al. (2007) Hsp40 molecules that target to the ubiquitin-proteasome system decrease inclusion formation in models of polyglutamine disease. Mol Ther. 15: 1100-5. 6. Keravala, A. et al. (2008) Long-term transgene expression in mouse neural progenitor cells modified with phiC31 integrase. J Neurosci Methods. 173: 299-305. 7. Zhou, H. et al. (2009) Dynamic near-infrared optical imaging of 2-deoxyglucose uptake by intracranial glioma of athymic mice. PLoS One. 4: e8051.
USBio References
No references available
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