MIP-1 alpha is also known as CCL3. MIP-1 alpha has a molecular weight of approximately 7.8kD and is produced by activated macrophages. MIP-1 alpha has been shown to be involved in the inflammatory response of blood monocytes and tissue macrophages.
Applications
Suitable for use in FLISA, Western Blot and Functional Assays. Other applications not tested.
Recommended Dilutions
Optimal working dilutions to be determined by the researcher.
Recommended Secondary Reagents
Sheep anti Rabbit IgG: HRP
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Note: Applications are based on unconjugated antibody.
Immunogen
Recombinant protein corresponding to rat MIP-1 alpha, expressed in E.coli.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by affinity chromatography from whole serum.
Specificity
Recognizes rat macrophage inflammatory protein-1 alpha (MIP-1 alpha).
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