MIP-1 alpha, also known as CCL3, has a molecular weight of ~7.8kD, which is produced by activated macrophages. MIP-1 alpha has been shown to be involved in the inflammatory response of blood monocytes and tissue macrophages.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Optimal working dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS, 0.1% BSA. For long term storage add 0.09% sodium azide. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant rat MIP-1 alpha, expressed in E.coli.
Form
Supplied as a lyophilized powder from PBS. Reconstitute with 1ml sterile PBS, 0.1% BSA. Labeled with Biotin.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes rat macrophage inflammatory protein-1 alpha (MIP-1 alpha).
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