Suitable for use in Flow Cytometry (membrane permeabilization is recommended) and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution
Immunohistochemistry (frozen sections): 1:25 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Mouse lymph node stroma
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes an intracellular antigen of mouse macrophages and monocytes. Reacts strongly with macrophages in lymphoid organs such as tingible body macrophages in T-cell dependent areas. Reacts on all mouse strains tested.
References
1. Kraal, G., et al., J. Immunol. 26: 653–661 (1987).
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