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M2346-30 Rabbit Anti-MAP1LC3C (Microtubule Associated Proteins 1A/1B Light Chain 3C, Autophagy Related Protein LC3C, Autophagy Related Ubiquitin-like Modifier LC3C, LC3C, LC3-like Protein 2, MAP1 Light Chain 3-like Protein 2, MAP1 Light Chain 3-like Protein 3, MAP1A/MAP1BLC3C, Microtubule Associated Protein 1 Light Chain 3 gamma) (Not for Export EU)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Serum
Applications
IF IHC WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

Probably involved in formation of autophagosomal vacuoles (autophagosomes). The subunit is 3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins.

Applications
Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Immunohistochemistry: 1:100-1:1000 Western Blot: 1:100-1:1000 Immunofluorescence: 1:100-1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to CQEEVAGIRAKF at the N-terminal of human MAP1LC3 C conjugated to Blue carrier Protein. Peptide Sequence Homology: monkey (Macaca mulatta) and canine.
Form
Supplied as a lyophilized powder. Reconstitute with 100ul of sterile ddH2O.
Purity
Serum
Specificity
Recognizes human MAP1LC3C. Does not recognize forms MAP1LC3A and MAP1LC3B.
References
1. Greenberg JT. Dev Cell. 8(6):799-801 (2005). 2. Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10 (2005). 3. Levine B. Cell. 120(2):159-62 (2005). 4. Tanida I., et al. J. Biol. Chem. 279:36268-36276 (2004). 5. Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48 (2005). 6. Tanida I., et al. Int. J. Biochem. Cell Biol. 36:2503-2518 (2004).
USBio References
No references available
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