Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Immunohistochemistry: ~0.5-1ug/ml (or 1:1000-1:2000). For optimal results, tissues or cytospin preparations should be fixed in Carnoy’s Fluid (60% ethanol, 30% chloroform, 10% glacial acetic acid). Not recommended for use on formaldehyde fixed tissue. May be used on thin sections prepared for ultrastructural analysis after fixation of tissue in a modified Karnovsky’s reagent (Lab Invest. (1988). 58:682–691). Western Blot: 0.5ug/ml detected tryptase in 10ug of human fetal skin lysate. Optimal dilutions to be determined by the researcher.

Mast cells, basophils Human fetal skin lysate

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Supplied as a liquid in Tris-Glycine, pH 7.4, 0.05% sodium azide.

Purified by Protein G affinity chromatography.

Recognizes human Mast cell tryptase. Will show reactivity to basophils but to a lesser degree.