MHC Class 1 is expressed at varying levels on the majority of nucleated cells. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the histocompatibility 2 (H-2) region.
Applications
Suitable for use in Flow Cytometry and Immunoprecipitation. Other applications have not been tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Hybridoma
X63 myeloma cells with spleen cells from C1D mice.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Purified H-2Kb and H-2Db MHC-I molecules.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Protein A affinity chromatography from tissue culture supernatant.
Specificity
Recognizes the monomorphic epitope present on mouse MHC Class 1. The epitope recognised is conformation dependent and is reported to be phylogenetically conserved. Reactivity has been observed with some canine samples suggesting that this may recognize a polymorphic epitope of canine MHC class I. Species Crossreactivity: rat, guinea pig, sheep, bovine, porcine, human, hamster.