This antibody recognizes a 60 kDa nonglycosylated protein component of mitochondria found in human cells. It can be used to stain mitochondria in cell, tissue or biochemical preparations and can be used as a marker of the mitochondria in subcellular fractions.

Suitable for use in Indirect Immunofluorescence, Immunohistochemistry, and Immunoprecipitation. Can also be used for isolation of intact mitochondria from freeze/thaw lysates of living cells via conjugation to magnetic beads. Other applications have not been tested.

Indirect Immunofluorescence: 1:10-1:50, 30-100ul per slide well Immunohistochemistry (paraffin-embedded tissues): 1:80 Using heart ventricle epithelium pretreated with EDTA, pH 8.0. Detection with HRP-DAB. Immunoreactivity is seeing the circular-granular layer surrounding the nucleus of the cells. Requires citric acid/microwave antigen retrieval; enhanced ABC or amplified detection systems recommended. Immunohistochemistry: 1:100. Works best with organic fixatives (acetone, methanol, etc.). 2% PFA for 10-15 minutes. can be used but stronger use of formalin or 4% PFA limits staining. Optimal dilutions to be determined by the researcher.

Positive Control: COS7 cells, heart ventricle

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Surface of intact human mitochondria

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.

Purified by ammonium sulfate precipitation from hybridoma supernatant.

Recognizes human mitochondria. Gives mitochondrial staining on all human cell types. This pattern is best described as a spaghetti- like staining pattern in fibroblasts and a perinuclear large speckled pattern in lymphonoid cells. Recognizes a 65kD protein by immunoprecipitation. Does not cross-react with rat and mouse. Epitope: surface of intact mitochondria.