USBio Logo

M9601-34A Rabbit Anti-Myc-Tag (c-myc, myc-1 epitope tag)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
P01106
Isotype
IgG
Grade
Affinity Purified
Applications
IC WB
Crossreactivity
Hu
Accession #
NM_002467.3
Shipping Temp
Blue Ice
Storage Temp
-20°C

Applications
Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 0.5-2ug/ml detected a Myc epitope tagged protein in a transfected Cos-1 cell lysate. 1ug/ml of a previous lot detected endogenous Myc in HeLa nuclear extract. A lysate of transfected Cos-1 cells was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Myc Tag (0.5ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Myc epitope tagged protein (~66kD) Immunocytochemistry: Reported to detect Myc-tagged proteins in transfected cells Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to residues 409-420 of human Myc (MEQKLISEEDLN)
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes and is specific for human Myc and recombinant proteins containing the Myc epitope tag.
References
1. Evan, et al., Mol. Cell. Biol. 12: 3610-3616, 1985. 2. Tian, L, et al., J. Biol. Chem. 275: 7854-7861, 2000.
USBio References
No references available
Conjugates
Pricing
Order
Proceed to Checkout
Cart Summary
ProductSizeListYour PriceQtyExt Price
Subtotal:Subtotal:
Subtotal:Subtotal:
Total Coupon Savings:Total Coupon Savings:()
Your cart is currently empty.
- Coupon Code
Recently Viewed
Contact Us

Visit our technical library or contact our support staff to answer your questions.

Telephone:
1-800-520-3011

Library | Contact

Distributors

For customers outside of the United States, please use one of our many distributors.

View Distributors

Payment Methods

We accept the following payment methods as well as pay-by-invoice.

MasterCard Visa PayPal
© 2023-2024 United States Biological - All Rights Reserved