Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:500-1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthesized non-phosphopeptide derived from mouse Myosin Light Chain 2 around the phosphorylation site of serine 18 (A-T-SP-N-V)
Form
Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide and 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Detects endogenous levels of total Myosin Light Chain 2 protein.
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