GDF8, also known as Myostatin, belongs to the TGF-beta superfamily. It is synthesized as an inactive proprotein with the Nterminal proregion and the Cterminal mature bioactive region. The GDF8 proregion is capable of associating with active GDF8 with high affinity and is a potent GDF8 antagonist.
Applications
Suitable for use in Western Blot and Direct ELISA. Other applications not tested.
Recommended Dilutions
Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to Asn25-Ser376 of mouse GDF-8 expressed in NS0 cells. Swiss/UniProt: O08689.
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with sterile PBS to 0.5mg/ml.
Purity
Purified by Protein G affinity chromatography from culture supernantant.
Specificity
Recognizes mouse, human and rat GDF8 in direct ELISA and WB. Shows ~30% cross-reactivity with recombinant human, mouse and rat GDF11 in WB. Shows <1% cross-reactivity with recombinant mouse GDF1, GDF3, GDF5, GDF6 and GDF9 in direct ELISA.