USBio Logo

N0531 Rabbit Anti-NAC, alpha (Nascent polypeptide-associated complex)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
Q9H009
Isotype
IgG
Grade
Affinity Purified
Applications
WB
Crossreactivity
Hu Mo Rt
Gene ID
342538
Shipping Temp
Blue Ice
Storage Temp
-20°C

Nascent polypeptide-associated complex (NAC) is an abundant cytosolic protein involved in cotranslational targeting of polypeptides to the endoplasmic reticulum (ER) membrane. NAC preparations consist of two copurifying polypeptides termed alpha- and beta-NAC that form a very stable complex in vivo and in vitro, and a functional complex can be reconstituted from the individual subunits. A dissection of the contributions of the individual subunits to NACs function revealed that both subunits are in direct contact with nascent polypeptide chains on the ribosome and that both contribute to the prevention of inappropriate interactions. Alpha-NAC is a coactivator of AP-1-mediated transcription. The alpha-NAC protein functions as a transcriptional coactivator by potentiating the activity of the chimeric Gal4-VP16 activator and of c-Jun homodimers. alpha-NAC binds to and regulates FADD function, which is a critical mediator of signal transduction pathways activated by several members of the TNF receptor gene superfamily. Human alpha-NAC is decreased in patients with Alzheimer’s disease and Down syndrome.

Applications
Suitable for use in Western Blot. Other applications have not been tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Positive Controls
Fetal mouse brain, rat brain homogenates and human NGP96 cell lysate.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
C-terminal region of human alpha-NAC
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes the human alpha-NAC protein at ~23kD. Species Crossreactivity: mouse and rat.
References
1. Beatrix, B., et al., J. Biol. Chem. 275: 37,838-37,845 (2000). 2. Moreau, A., et al., Mol. Cell Biol. 18: 1312-1321 (1998). 3. Quelo, I., et al., Biochemistry 43: 2906-2914 (2004). 4. Quelo, I., et al., Gene Expr. 10: 255-262 (2002). 5. Yotov, W.V., et al., Mol. Cell Biol. 18: 1303-1311 (1998). 6. Stilo, R., et al., Biochem. Biophys. Res. Commun. 303: 1034-1041 (2003). 7. Kim, S.H., et al., J. Investig. Med. 50: 293-301 (2002).
USBio References
No references available
Conjugates
Pricing
Order
Proceed to Checkout
Cart Summary
ProductSizeListYour PriceQtyExt Price
Subtotal:Subtotal:
Subtotal:Subtotal:
Total Coupon Savings:Total Coupon Savings:()
Your cart is currently empty.
- Coupon Code
Recently Viewed
Contact Us

Visit our technical library or contact our support staff to answer your questions.

Telephone:
1-800-520-3011

Library | Contact

Distributors

For customers outside of the United States, please use one of our many distributors.

View Distributors

Payment Methods

We accept the following payment methods as well as pay-by-invoice.

MasterCard Visa PayPal
© 2023-2024 United States Biological - All Rights Reserved