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N0536-07A Rabbit Anti-NBC1 (Na, HCO3 Cotransporter 1, pNBC) (Not for Export EU)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Rat
Isotype
IgG
Grade
Serum
Applications
E WB
Crossreactivity
Rt
Accession #
AF027362
Shipping Temp
Blue Ice
Storage Temp
-20°C

Bicarbonate, along with CO2, is the major pH buffer of biological fluids. A great majority of HCO3-reabsorption occurs via trans-cellular coupling of the luminal Na-H+-exchanger 3 and Na+-H+-ATPase with the basolateral Na+-HCO3- cotransporters (NBC). Several related proteins constitute the emerging NBC family (NBC1-3) of membrane cotransporters that are found in a variety of epithelial and non-epithelial tissues, and may be tissue specific. Physiologically, NBC is electrogenic, Na+ and HCO3- dependent, Cl- independent, and inhibited by stilbenes (DIDS and SITS). The NBC family of proteins are 30-35% related to anion exchangers (AE2 and AE3; SLC4A1-SLC4A3) and display the same protein topology: (a) At least 10 TM domains with both the N and C-termini predicted to be intracellular, (b) presence of a large, glycosylated, extracellular loop between TM5 and TM6; and (c) the lysine residues are conserved at predicted DIDS-reactive sites. NBC1 (human, rat, mouse 1035 aa, also called NBC-1A/1B, hkNBC, rkNBC, pNBC, hhNBC, SLC4A4) was initially cloned from human Kidney. NBC1 is 30-35% identical with AEs. It is strongly expressed in the kidney and pancreas. The rat kidney NBC1 (rkNBC1; 1035 aa) is 86% identical to hkNBC1. Pancreatic NBC (pNBC/hhNBC/SLC4A5, human 1079 aa) has a unique n-terminal 85 aa sequence replacing the 41-aa in kNBC. pNBC is also more widely expressed (pancreas, thyroid, heart, and brain).

Applications
Suitable for use in Western Blot and ELISA. Other applications not tested.
Recommended Dilution
Western Blot: 1-10ug/ml; using Chemiluminescence technique. ELISA: 1:10,000-1:100,000; Using 50-100ng of control peptide/well. See N0536-08. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
20aa peptide from rat NBC1, conjugated to KLH. Epitope Location: ~C-terminus, Cytoplasmic domain. No significant sequence homology was found with NBC2 or NBC3.
Form
Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Purity
Serum
Specificity
Recognizes rat NBC1. Antibodies will recognize both the kidney and pancreatic/heart isoforms of NBC1. Species Sequence Homology: Mouse - 100%; Human - 85%; Frog, rabbit - 80%.
References
Romero MF (1998) AJP 274, F425-F432 Burnham CE (1998)|Burnham CE (1997) JBC 272, 19111-19114|Abuladze N (1998) JBC 273; 17689-17695|Solemani M (2000) Kidney Intl. 57, 371-384 (review)|Romero MF & Boron WF (1999) Ann. Rev. Physiol. 61, 699-723 (review)|Velic A. 2004, Am. Soc. Nephrol., 15: 967 - 977|Robey RB 2002, Journal of Membrane Biology
USBio References
No references available
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