Mammalian NHE (Na+/H+ Exchanger) is an integral membrane protein that is known to regulate intracellular pH by removing a proton in exchange for an extracellular sodium ion. There are nine known isofoms of NHE. The best characterized is NHE1, involved not only in intracellular pH control, but cell-volume control, cytoskeletal organization, heart disease and cancer. Transport by the NHE plays a pivotal role in the damage caused to the human myocardium during and following a myocardial infarction, and it is considered to represent a key step in the oncogenic transformation of cancerous cells. NHE comprises an N-terminal membrane domain that transports ions, and a C-terminal cytoplasmic domain that regulates activity and mediates cytoskeletal interactions. NHE2 - NHE5 isoforms are localized to the plasma membrane but exhibit greater tissue- specificity. NH2 and NH3 are more highly expressed in the kidney and intestine. Structurally, a single transmembrane segment from NHE1 has been solved, and the structure of bacterial Na+/H+ antiporter NhaA has been elucidated.

Suitable for use in Western Blot. Works poorly in Immunohistochemistry and Immunocytochemistry. Not suitable for use in Immunoprecipitation. Other applications not tested.

Western Blot: 1:500 detected NHE1 on 10ug of human kidney lysates. Human kidney lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with N2015-02 (1:500). Proteins were visualized using an IgG (HRP) Gt xMo* and a chemiluminescence detection system. Optimal dilutions to be determined by the researcher.

I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. 

Note: Applications are based on unconjugated antibody.

MBP fusion protein containing the entire C-terminal, hydrophilic domain of porcine NHE1.

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.

Purified by Protein A affinity chromatography.

Recognizes Na+/H+ exchanger, isoform NHE1 at ~100-110kD. Species Crossreactivity: All vertebrates including fish, amphibian, avian and mammalian.