USBio Logo

N2565-30B Rabbit Anti-Nicotinic Receptor, beta 2

Specifications
Clone Type
Polyclonal
Host
Rabbit
Isotype
IgG
Grade
Highly Purified
Applications
E IC WB
Shipping Temp
Blue Ice
Storage Temp
-20°C

Acetylcholine receptors (AChRs) mediate synaptic transmission at the neuromuscular junction. The channel-linked AChR that mediates rapid, excitatory actions of acetylcholine is called nicotinic AChR (nAChR) because it can be activated by nicotine. The non-channel linked AChR that medicates the slow actions of acetylcholine, which can be either inhibitory or excitatory, is called muscarinic AChR (mAChR) because it can be activated by muscarine. The nAChRs fall into two subclasses, the muscle-type and the neuronal-type. The muscle-type receptor is a pentameric glycoprotein of five membrane spanning subunits, (2 alpha 1’s, beta 1, gamma or epsilon and delta) which form a ligand gated ion channel. The neuronal nAChR is also a pentamer, but unlike the muscle-type, is made up of only two general types of subunits, alpha and beta of which multiple subtypes have been described (alpha 2 to alpha 8 and beta 2 to beta 4).

Applications
Suitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested.
Recommended Dilutions
Western Blot (Tissue Homogenates): 25ug/ml detects a band at ~60kD Immunocytochemistry (ABC): 20ug/ml. Cells shoud be fixed using PLP. Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Peptide analog of the carboxyl terminal aa493-502 of the nicotinic beta2 receptor, conjugated to a carrier protein.
Form
Supplied as a liquid in PBS, pH 7.2.
Purity
Purified by precipitation, dialysis and chromatography.
Specificity
Recognizes Nicotinic beta2 receptor (493-502) and the reactivity of Nicotinic beta2 receptor is ~90%. Does not react to Nicotinic alpha3 receptor, Nicotinic alpha4 receptor, Nicotinic alpha5 receptor, Nicotinic alpha7 receptor, Nicotinic beta3 receptor or the Nicotinic beta4 receptor. Specific for the COOH terminal of nicotinic beta2 receptor.
Conjugates
Pricing
Order
Proceed to Checkout
Cart Summary
ProductSizeListYour PriceQtyExt Price
Subtotal:Subtotal:
Subtotal:Subtotal:
Total Coupon Savings:Total Coupon Savings:()
Your cart is currently empty.
- Coupon Code
Recently Viewed
Contact Us

Visit our technical library or contact our support staff to answer your questions.

Telephone:
1-800-520-3011

Library | Contact

Distributors

For customers outside of the United States, please use one of our many distributors.

View Distributors

Payment Methods

We accept the following payment methods as well as pay-by-invoice.

MasterCard Visa PayPal
© 2023-2024 United States Biological - All Rights Reserved