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O7050-05B Rat Anti-ORC2 (Origin Recognition Complex Subunit 2)

Specifications
References
Clone Type
Monoclonal
Host
Rat
Source
Human
Swiss Prot
Q13416
Isotype
IgG2a
Clone Number
7H46(3G6)
Grade
Ascites
Applications
E IF IP WB
Crossreactivity
Hu Mk
Gene ID
4999
Shipping Temp
Blue Ice
Storage Temp
-20°C

The origin recognition complex (ORC) is a highly conserved heterohexameric protein complex that associates with DNA at or near sites of initiation of DNA replication. All six ORC subunits are essential for initiation of DNA replication(1-3), and ORC may be involved in regulation of gene expression in response to stress(4). ORC binding to DNA permits the ordered binding of other proteins such as cdc6 and MCMs to form pre-replication complexes (Pre-RCs). Pre-RCs form between telophase and early G1 phase of the cell cycle and are inactivated at the onset of DNA synthesis, allowing coordinated regulation of DNA replication and cell division(5). Modification of one or more of the six ORC subunits may be responsible for its inactivation during S phase, but the chromatin binding behavior of the ORC subunits during the cell division cycle is still under investigation(6-7).

Applications
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunofluorescence. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (IF-IC): 1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to full length human ORC2.
Form
Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Purity
Ascites
Specificity
Recognizes endogenous levels of total human ORC2 protein at ~78kD. Does not crossreact with other ORC subunits. Species Crossreactivity: monkey.
References
1. Machida, Y.J. et al. (2005) J. Biol. Chem. 280, 27624–27630. 2. Baltin, J. et al. (2006) J. Biol. Chem. 281, 12428–12435. 3. Gibson, D.G. et al. (2006) Genes Cells 11, 557–573. 4. Ramachandran, L. et al. (2006) FEMS Yeast Res. 6, 763–776. 5. Rowles, A. and Blow, J.J. (1997) Curr Opin Genet Dev 7, 152-157. 6. DePamphilis, M.L. (2003) Gene 310, 1-15. 7. McNairn, A.J. et al. (2005) Exp Cell Res 308, 345-356.
USBio References
No references available
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