PEAR1 (platelet endothelial aggregation receptor 1; also MEGF12) is a 150kD member of the MEGF family of proteins. It is expressed principally on platelets and endothelium, and undergoes phosphorylation in response to platelet activation and adhesion. Mature human PEAR1 is a type I transmembrane protein that is 1017 amino acids (aa) in length. It contains a 735 aa extracellular region (aa 21-755) and a Pro-rich 261 aa cytoplasmic domain. The extracellular region shows one EMI segment (aa 25-103) followed by nine EGF-like repeats (aa 104-692). There is one potential alternate start site at Met376.
Applications
Suitable for use in Direct ELISA, Western Blot and Flow Cytometry. Other applications not tested.
Recommended Dilution
Flow Cytometry: 10ug/ml. Use 10ul to label 1-5x10e5 blood derived CD41+ platelets. Direct ELISA: 0.5-1.0ug/ml. Detection limit for rhPEAR1 is~ 0.3ng/well. Western Blot: 0.1-0.2ug/ml. Detection limit for rhPEAR1 is ~ 1ng/lane and 10 ng/lane under non-reducing and reducing conditions, respectively. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant corresponding to aa21-754 extracellular domain of human PEAR1, expressed in CHO cells. (Q5VY43).
Form
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human PEAR1. Species sequence homology: mouse and canine.
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