Pepsinogen I

Clone Type

Monoclonal

Host

Mouse

Source

Human

Isotype

IgG1

Clone Number

8.BG.1 (99/12)

Grade

Affinity Purified

Applications

E IHC RIA

Crossreactivity

Shipping Temp

Blue Ice

Storage Temp

-20°C

Pepsinogen I, a zymogen, or proenzyme secreted by Chief cells in the stomach, is cleaved to form pepsin both in an autocatalytic fashion and by pepsin itself. In humans there are two related forms of pepsin, Pepsinogen I, also known as Pepsinogen A, and Pepsinogen II, also known as Pepsinogen B or progastricsin.

Applications

Suitable for use in ELISA, Immunohistochemistry and RIA. Other applications have not been tested.

Recommended Dilutions

Immunohistochemistry: Paraffin sections. Requires HIER using sodium citrate buffer pH6.0 prior to staining. Optimal dilutions to be determined by the researcher.

Affinity Constant

4x10e10L/mol

Recommended Secondary Antibodies

I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

Human pepsinogen I (native).

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Purity

Purified by Protein A affinity chromatography from tissue culture supernatant

Specificity

Recognizes human pepsinogen I, both zymogen and proenzyme.

References

US Biological application references: 1. Lieu, J.E.C., et al., (2005) Otolaryngology-Head and Neck Surgery 133:357-361 2. Farhath, S. et al., (2008) Pediatrics 121:e253-e259. 3. Fujita Y., et al., Gastric Cancer 19(1):192-7 (2016). 4. Hidaka Y., et al., Human Pathology 44: 2438-48 (2013). 5. Sakamoto H., et al., Journal of Gastroenterology 46: 620-8 (2011). 6. Nakajima T., et al., Pathol. Inter. 66(9): 518-23 (2016). 7. Mamat O., et al., Histopathology, March 17(2016). 8. Mitsuishi T., et al., PLoS One 12(4): e0174985 (2017). 9. Chiba T., et al., Dig Endosco. 28(7): 722-30 (2016).

USBio References

US Biological application references: 1. Lieu, J.E.C., et al., (2005) Otolaryngology-Head and Neck Surgery 133:357-361 2. Farhath, S. et al., (2008) Pediatrics 121:e253-e259.

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P3272-06 Mouse Anti-Pepsinogen I

Clone Type

Host

Source

Isotype

Clone Number

Grade

Applications

Crossreactivity

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References

USBio References

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