The peroxidase-antiperoxidase (PAP) approach utilizes a complex of enzyme and antibody to facilitate amplification of signal, indirectly detecting the amount of primary antibody used in an assay. Generated in Rabbit, the PAP complex can be utilized in any assay that utilizes a rabbit primary antibody. With the addition of a secondary antibody that recognizes rabbit, the reporter PAP complex allows for amplification of the luminescent signal (as three molecules of enzyme are bound to every two immunoglobulins).
Applications
Suitable for use in Immunohistochemistry and Peroxidase Enzymatic Labeling. Other applications have not been tested
Recommended Dilutions
Note: Recommended that further dulutions be made with 5% normal serum from same host bridging antibody. Optimal dilutions to be determined by researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
Immunogen
Horseradish peroxidase
Form
Supplied as a liquid in PBS, pH 7.2. No stabilizing proteins or preservatives added. Labeled with HRP.
Purity
Purified by delipidation, salt fractionation and ion exchange chromatography.
Specificity
Recognizes peroxidase. Assay by IEP resulted in a single precipitin arc against anti-rabbit serum and anti-peroxidase.
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