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P4077 Rabbit Anti-Phosphothreonine

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC IP WB
Shipping Temp
Blue Ice
Storage Temp
-20°C

Protein phosphorylation is a complex biochemical process that ultimately influences the mechanism by which a cell responds to a particular stimulus. A class of enzymes referred to as protein kinases regulates this process; these enzymes catalyze the transfer of the gama-P of ATP to a recipient protein that acts as a substrate. This post-translational modification is primarily directed onto serine and threonine amino acid residues within a protein. It has been noted that changes in the phosphorylation status of these phosphoproteins can alter the biological function of a protein. Additionally, aberrations in signal transduction cascade have been linked to a number of diseases including cancer, diabetes, heart disease, inflammation and neurological disorders.

Applications
Suitable for use in Western Blot, ELISA, Immunohistochemistry and Immunoprecipitation. Other applications have not been tested.
Recommended Dilutions
Western Blot: 2ug/ml; Mouse brain lysate. Incubate at RT for 4 hrs. ELISA: 0.5ug/ml (kinase assay). Binding of Ly-pT-Gly-HRP (0.2ug/100ul/well) to an anti-phosphothreonine coated microplate (0.5ug/100ul/well) is inhibited by 95% with the addition of 2-10ug/ml anti-phosphothreonine. No inhibition is evident by inhibition of phosphoserine, phosphotyrosine or threonine at concentrations of up to 1mg/ml. Immunohistochemistry: paraffin sections Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Phosphothreonine conjugated to KLH and phosvitin.
Form
Supplied as a liquid in PBS, pH 7.0, 0.01% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes phosphothreonine, peptidyl-phosphothreonine and threonine-phosphorylated proteins.
References
1. Frackelton, A.R., et al., Mol. Cell. Biol. 3: 1343 (1983). 2. Edelman, A.M., et al., Annu. Rev. Biochem. 56: 567 (1987). 3. Hunter, T., Cell 50: 823 (1987). 4. Glenny, J.R., et al., J. Immunological Meth. 109: 277 (1988). 5. Sengupta, A., et al., Proc. Natl. Acad. Sci. USA 85: 8062 (1988). 6. Heffetz, D., et al., Methods in Enzymology 210: 44 (1991). 7. Hunter, T., Methods in Enzymology 200: 3 (1991). 8. Rintamäki, E., et al., J. Biol. Chem. 272(48): 30,476–30,482 (1997). 9. Jin, D.Y., et al., Cell. 93: 81–91 (1998). 10. Reilein, A.R., et al., J. Biol. 142(3): 803–813 (1998). 11. Fleming, I.N., et al., J. Biol. Chem. 274(18): 12,753–12,758 (1999).
USBio References
No references available
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