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P4214-10X-PE Mouse Anti-Phosphatase, Alkaline, Placental (Alkaline phosphatase, placental type, Alkaline phosphatase Regan isozyme, ALP, FLJ61142, PALP, Placental alkaline phosphatase 1, PLAP, PLAP-1) (BSA & Azide Free) (PE)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
PE
Isotype
IgG2a,k
Clone Number
0.N.515
Grade
Affinity Purified
Applications
FLISA
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free

Unlike germ cell tumors, PLAP-positive somatic cell tumors uniformly express epithelial membrane antigen (EMA).

Applications
Suitable for use in FLISA. Not suitable for use in Immunohistochemistry. Other applications have not been tested.
Recommended Dilution
FLISA: Coating antibody Optimal dilutions to be determined by the researcher.
Positive Control
Placenta
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
HEp-2 cells expressing Placental Alkaline Phosphatase (PLAP). MW of Antigen: 60-70kD. Cellular Localization: Cell membrane.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human placental alkaline phosphatase (PLAP). Useful in the identification of germ cell tumors.
References
1. Durbin, H., et al., (1988) Int. J. Cancer: Suppl. 2: 50-58. 2. Durbin, H., et al., (1988) Int. J. Cancer: Suppl. 2: 59-66.
USBio References
No references available
Conjugates
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