USBio Logo

P4256-31A Sheep Anti-Plasminogen Activator Inhibitor Type 1 (PAI 1) (HRP)

Specifications
Clone Type
Polyclonal
Host
Sheep
Source
Human
Swiss Prot
Q8NC51
Conjugate
HRP
Isotype
IgG
Grade
Affinity Purified
Applications
E
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

Type I plasminogen activator inhibitor, PAI 1, is a 50kD single- chain glycoprotein which belongs to the serine protease inhibitor (SERPIN) family of proteins. The concentration of PAI 1 in normal human plasma is very low, with an average concentration of ~20ng/ml. The plasma concentrations of PAI 1 can be affected by a number of factors including diurnal variations, age, sex, pregnancy, obesity and exercise status. PAI 1 is synthesized by various cell types including endothelial cells, hepatocytes, vascular smooth muscle cells, fibroblasts, mesothelial cells, granulosa cells and malignant cell lines. It is also found in the alpha-granules of platelets as well as plasma. PAI 1 exists in at least three different conformations, including an active form with a half-life of ~1-3 hours, a latent form and a proteolytically or oxidatively inactivated form. The plasma glycoprotein vitronectin has been shown to bind active PAI 1 and stabilize it in its active conformation, extending its functional half-life to greater than 24 hours. Little specific binding of the latent or inactive forms of PAI 1 to vitronectin occurs. PAI 1 is considered to be the primary regulator of plasminogen activation in vivo. It is the major physiologic inhibitor of both the single chain and two-chain forms of tPA, being able to inhibit the single-chain form at least 10000 times greater than other PAIs.

Applications
Suitable for use in ELISA and Immunoelectrophoresis. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Rz Ratio (Reinheitszahl, A403/A280)
0.41
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant Plasminogen Activator Inhibitor Type 1 (PAI 1) from bacterial extracts.
Form
Supplied as a liquid in buffered stabilizer solution, 50% glycerol. Labeled with Horseradish Peroxidase (HRP).
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Plasminogen Activator Inhibitor Type 1.
Conjugates
Pricing
Order
Proceed to Checkout
Cart Summary
ProductSizeListYour PriceQtyExt Price
Subtotal:Subtotal:
Subtotal:Subtotal:
Total Coupon Savings:Total Coupon Savings:()
Your cart is currently empty.
- Coupon Code
Recently Viewed
Contact Us

Visit our technical library or contact our support staff to answer your questions.

Telephone:
1-800-520-3011

Library | Contact

Distributors

For customers outside of the United States, please use one of our many distributors.

View Distributors

Payment Methods

We accept the following payment methods as well as pay-by-invoice.

MasterCard Visa PayPal
© 2023-2024 United States Biological - All Rights Reserved