BioAssay™ Total Protein Extraction Kit is an excellent tool for the initial purification and preparation of proteins from any tissues and cells. Total protein isolated by this kit is native and can be used for downstream applications including SDS-PAGE, Western Blot, gel mobility shift, protein assays, DNA-protein interaction, enzymatic activity analysis, protein-protein interaction, and immunoprecipitation and tissue specific expression identification.

The kit is easy to use: no scraping, no freeze-thaw cycles, and no sonication.

The Total Protein Extraction Kit is composed of two buffers. Before isolation of total proteins from tissues or cells, simply dilute Buffer B buffer with Buffer A according to the protocol provided. The kit can be used for isolation of total proteins from 5g of tissue or 125 million cultured cells. Minimal 0.1g tissue or 2.5 million cells should be required

• Easy protocol • Isolate protein from large samples • Rapid isolation without freeze and thaw and sonication • Isolate protein either from tissues or from cultured cells • Proteins isolated by this kit are intact and could be over 200kD in mass.

Suitable for use in Western Blot, DNA-protein interaction, enzymatic activity analysis, protein-protein interaction, Immunohistochemistry, SDS-PAGE and Immunoprecipitation. Other applications not tested.

Minimum of 0.1g tissue or 2.5 million cells should be used.

P9087A: Buffer A, 13ml. Contains HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, Sodium OrthoVanadate.

P9087B: PI, 50X, 260ul. Contains a cocktail of protease inhibitors.

Store P9087A at 4°C and P9087B at -20°C. Stable for 6 months after receipt under proper storage conditions. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.